Generation and initial characterization of Pseudomonas stutzeri KC mutants with impaired ability to degrade carbon tetrachloride

@article{DelCSeplvedaTorres1999GenerationAI,
  title={Generation and initial characterization of Pseudomonas stutzeri KC mutants with impaired ability to degrade carbon tetrachloride},
  author={Lycely Del C. Sep{\'u}lveda-Torres and Narayanan Rajendran and Michael J. Dybas and Craig S. Criddle},
  journal={Archives of Microbiology},
  year={1999},
  volume={171},
  pages={424-429}
}
Abstract Under iron-limiting conditions, Pseudomonas stutzeri KC secretes a small but as yet unidentified factor that transforms carbon tetrachloride (CT) to CO2 and nonvolatile products when activated by reduction at cell membranes. Pseudomonas fluorescens and other cell types activate the factor. Triparental mating was used to generate kanamycin-resistant lux::Tn5 recombinants of strain KC. Recombinants were streaked onto the surface of agar medium plugs in microtiter plates and were then… Expand
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