Generation and functional analysis of zinc finger nucleases.

  title={Generation and functional analysis of zinc finger nucleases.},
  author={Toni Cathomen and David J Segal and Vincent Brondani and Felix M{\"u}ller-Lerch},
  journal={Methods in molecular biology},
The recent development of artificial endonucleases with tailored specificities has opened the door for a wide range of new applications, including the correction of mutated genes directly in the chromosome. This kind of gene therapy is based on homologous recombination, which can be stimulated by the creation of a targeted DNA double-strand break (DSB) near the site of the desired recombination event. Artificial nucleases containing zinc finger DNA-binding domains have provided important proofs… CONTINUE READING

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