Generation and characterization of novel monoclonal antibodies recognizing UBXD1.
@article{Ramkumar2009GenerationAC, title={Generation and characterization of novel monoclonal antibodies recognizing UBXD1.}, author={P. Ramkumar and Bennett A Smith and Anu C Akinbamidele and J. Kapcia and S. Beauparlant and D. Haines}, journal={Hybridoma}, year={2009}, volume={28 6}, pages={ 459-62 } }
UBXD1 is a recently identified adaptor for p97, a highly abundant and conserved member of the AAA family of ATPase that plays pivotal roles in a multitude of cellular processes involving the ubiquitin-proteasome pathway. Very little is known about the biochemical, cellular, and molecular functions of UBXD1. Here we report the generation of two mouse monoclonal antibodies, 5C3-1 and 2F8-24, that recognize UBXD1 using Western blotting, immunoprecipitation, and immunofluorescence.
Topics from this paper
One Citation
Protein Interaction Profiling of the p97 Adaptor UBXD1 Points to a Role for the Complex in Modulating ERGIC-53 Trafficking*
- Biology, Medicine
- Molecular & Cellular Proteomics
- 2012
- 29
- PDF
References
SHOWING 1-10 OF 11 REFERENCES
Identification and characterization of UBXD1, a novel UBX domain-containing gene on human chromosome 19p13, and its mouse ortholog.
- Biology, Medicine
- Biochimica et biophysica acta
- 2001
- 15
Ubxd1 is a novel co-factor of the human p97 ATPase.
- Biology, Medicine
- The international journal of biochemistry & cell biology
- 2008
- 42
UBXD7 Binds Multiple Ubiquitin Ligases and Implicates p97 in HIF1α Turnover
- Biology, Medicine
- Cell
- 2008
- 254
UBXD1 binds p97 through two independent binding sites.
- Biology, Medicine
- Biochemical and biophysical research communications
- 2009
- 32
A Novel Ubiquitination Factor, E4, Is Involved in Multiubiquitin Chain Assembly
- Biology, Medicine
- Cell
- 1999
- 1,018
VCIP135 acts as a deubiquitinating enzyme during p97–p47-mediated reassembly of mitotic Golgi fragments
- Biology, Medicine
- The Journal of cell biology
- 2004
- 147
- PDF
Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a.
- Biology, Medicine
- Biochemical and biophysical research communications
- 2003
- 53
Cdc48p(Npl4p/Ufd1p) binds and segregates membrane-anchored/tethered complexes via a polyubiquitin signal present on the anchors.
- Biology, Medicine
- Molecular cell
- 2007
- 89
Regulation of retrotranslocation by p97-associated deubiquitinating enzyme ataxin-3
- Biology, Medicine
- The Journal of cell biology
- 2006
- 187
- PDF
Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone.
- Biology, Medicine
- Molecular cell
- 2006
- 241