Generation and characterization of novel monoclonal antibodies recognizing UBXD1.

  title={Generation and characterization of novel monoclonal antibodies recognizing UBXD1.},
  author={P. Ramkumar and Bennett A Smith and Anu C Akinbamidele and J. Kapcia and S. Beauparlant and D. Haines},
  volume={28 6},
  • P. Ramkumar, Bennett A Smith, +3 authors D. Haines
  • Published 2009
  • Biology, Medicine
  • Hybridoma
  • UBXD1 is a recently identified adaptor for p97, a highly abundant and conserved member of the AAA family of ATPase that plays pivotal roles in a multitude of cellular processes involving the ubiquitin-proteasome pathway. Very little is known about the biochemical, cellular, and molecular functions of UBXD1. Here we report the generation of two mouse monoclonal antibodies, 5C3-1 and 2F8-24, that recognize UBXD1 using Western blotting, immunoprecipitation, and immunofluorescence. 
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