Generation and characterization of antibody against porcine interleukin-18.


The gene-encoding mature porcine interleukin-18 (pIL-18) was amplified by PCR and cloned into a prokaryotic expression vector pET-30a(+). The resulting recombinant plasmid pET-30a-pIL-18 was transformed into Escherichia coli. Expression of recombinant pIL-18 protein was induced by 1 mM isopropyl β-D-thiogalactoside at 37°C. The purified recombinant protein… (More)
DOI: 10.1089/hyb.2010.0095


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