Generation and characterization of an immunogenic dendritic cell population.

  title={Generation and characterization of an immunogenic dendritic cell population.},
  author={Stephan Gehring and Stephen H. Gregory and Philip Wintermeyer and Maryann San Martin and Costica Aloman and Jack R Wands},
  journal={Journal of immunological methods},
  volume={332 1-2},
Dendritic cells (DCs) capture, internalize and process antigens leading to the induction of antigen-specific immune responses. The aim of this study was to develop, implement and characterize an efficient approach for DC-based immunization. Dendritic cells were expanded in vivo by hydrodynamic delivery of a human flt3 ligand expression plasmid. Splenic DCs were isolated and purified with magnetic beads linked to hepatitis C virus (HCV) nonstructural protein-5 (NS5), anti-CD40 and/or LPS. The… CONTINUE READING


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