Generation and characterization of an attenuated mutant in a response regulator gene of Francisella tularensis live vaccine strain (LVS).

@article{SammonsJackson2008GenerationAC,
  title={Generation and characterization of an attenuated mutant in a response regulator gene of Francisella tularensis live vaccine strain (LVS).},
  author={Wendy Sammons-Jackson and K E McClelland and Jean N. Manch-Citron and Dennis W. Metzger and Chandra Shekhar Bakshi and Emilio Garcia and Amy Rasley and Burt E. Anderson},
  journal={DNA and cell biology},
  year={2008},
  volume={27 7},
  pages={
          387-403
        }
}
Francisella tularensis is a zoonotic bacterium that must exist in diverse environments ranging from arthropod vectors to mammalian hosts. To better understand how virulence genes are regulated in these different environments, a transcriptional response regulator gene (genome locus FTL0552) was deleted in F. tularensis live vaccine strain (LVS). The FTL0552 deletion mutant exhibited slightly reduced rates of extracellular growth but was unable to replicate or survive in mouse macrophages and was… 
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