Generation and application of a 293 cell line stably expressing bovine interferon-gamma.

Abstract

A stable mammalian cell line expressing highly active bovine interferon-gamma (BoIFN-γ) was generated using Flp recombinase-mediated integration. This recombinant 293 cell line (B1) efficiently secreted FLAG-tagged BoIFN-γ protein into the culture supernatant, as determined by ELISA and Western blot. The recombinant BoIFN-γ exhibited high anti-viral… (More)
DOI: 10.1016/j.pep.2014.04.012

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Cite this paper

@article{Xu2014GenerationAA, title={Generation and application of a 293 cell line stably expressing bovine interferon-gamma.}, author={Zhengzhong Xu and Fengli Shan and Fa Shan and Chuang Meng and Xiaohui Zhou and Xiaoming Zhang and Xiang Chen and Xin-an Jiao}, journal={Protein expression and purification}, year={2014}, volume={99}, pages={131-7} }