Generation and analysis of random point mutations in an antibody CDR2 sequence: many mutated antibodies lose their ability to bind antigen

@article{Chen1992GenerationAA,
  title={Generation and analysis of random point mutations in an antibody CDR2 sequence: many mutated antibodies lose their ability to bind antigen},
  author={Cui Cui Chen and Victoria A. Roberts and Marvin B. Rittenberg},
  journal={The Journal of Experimental Medicine},
  year={1992},
  volume={176},
  pages={855 - 866}
}
We have investigated the impact of mutations on the binding functions of the phosphocholine (PC)-specific T15 antibody in the absence of antigen selection pressure. The H chain complementarity determining region 2 (CDR2) sequence of T15 antibody was saturated with point mutations by in vitro random mutagenesis. From the mutant library, 289 clones were screened by direct DNA sequencing. The point mutations generated by this method were randomly distributed throughout the CDR2 region and included… CONTINUE READING

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Monoclonal antiidiotypes as a probe for the analysis of the diversity of antiphosphorylcholine antibodies

  • G. J. Hammerling, K. Wallich
  • In Protides of the Biological Fluids
  • 1980
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