Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection

@inproceedings{Bragg2012GenerationAC,
  title={Generation and Characterization of the Western Regional Research Center Brachypodium T-DNA Insertional Mutant Collection},
  author={Jennifer N. Bragg and Jiajie Wu and Sean P Gordon and Mara E. Guttman and Roger Thilmony and Gerard R. Lazo and Yong Qiang Gu and John P. Vogel},
  booktitle={PloS one},
  year={2012}
}
The model grass Brachypodium distachyon (Brachypodium) is an excellent system for studying the basic biology underlying traits relevant to the use of grasses as food, forage and energy crops. To add to the growing collection of Brachypodium resources available to plant scientists, we further optimized our Agrobacterium tumefaciens-mediated high-efficiency transformation method and generated 8,491 Brachypodium T-DNA lines. We used inverse PCR to sequence the DNA flanking the insertion sites in… CONTINUE READING

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