Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters

@article{Pavy2005GenerationAA,
  title={Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters},
  author={Nathalie Pavy and Charles Paule and Lee S. Parsons and John Allen Crow and M J Morency and Janice E. K. Cooke and James E. Johnson and Etienne Noumen and Carine Guillet-Claude and Yaron S. N. Butterfield and Sarah A. Barber and George S. Yang and Jerry Liu and Jeffery M Stott and Robert Kirkpatrick and Asim S. Siddiqui and Robert A. Holt and Marco A. Marra and Armand S{\'e}guin and Ernest F. Retzel and Jean Bousquet and John J. Mackay},
  journal={BMC Genomics},
  year={2005},
  volume={6},
  pages={144 - 144}
}
The sequencing and analysis of ESTs is for now the only practical approach for large-scale gene discovery and annotation in conifers because their very large genomes are unlikely to be sequenced in the near future. Our objective was to produce extensive collections of ESTs and cDNA clones to support manufacture of cDNA microarrays and gene discovery in white spruce (Picea glauca [Moench] Voss). We produced 16 cDNA libraries from different tissues and a variety of treatments, and partially… CONTINUE READING
Highly Influential
This paper has highly influenced a number of papers. REVIEW HIGHLY INFLUENTIAL CITATIONS