Generating new prions by targeted mutation or segment duplication

  title={Generating new prions by targeted mutation or segment duplication},
  author={Kacy R Paul and Connor G. Hendrich and Aubrey Waechter and Madison R Harman and Eric D. Ross},
  journal={Proceedings of the National Academy of Sciences},
  pages={8584 - 8589}
Significance Prions are self-propagating protein aggregates. We designed rational mutations in four nonprion proteins to examine possible mechanisms for how new prions could evolve. In each case, a small number of mutations were sufficient to cause the proteins to aggregate and, in two cases, to create prion activity. We likewise showed that simply creating tandem repeats of aggregation-prone segments within nonprion proteins can be sufficient to create prion activity, suggesting that such… 

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