Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells

Abstract

Human pluripotent stem cells (hPSCs) provide a valuable model for the study of human development and a means to generate a scalable source of cells for therapeutic applications. This protocol specifies cell fate efficiently into cardiac and endothelial lineages from hPSCs. The protocol takes 2 weeks to complete and requires experience in hPSC culture and… (More)
DOI: 10.1038/nprot.2016.153

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