Generating gene knockout rats by homologous recombination in embryonic stem cells

@article{Tong2011GeneratingGK,
  title={Generating gene knockout rats by homologous recombination in embryonic stem cells},
  author={Chang Tong and Guanyi Huang and Charles E Ashton and Ping Li and Qi-Long Ying},
  journal={Nature Protocols},
  year={2011},
  volume={6},
  pages={827-844}
}
We describe here a detailed protocol for generating gene knockout rats by homologous recombination in embryonic stem (ES) cells. This protocol comprises the following procedures: derivation and expansion of rat ES cells, construction of gene-targeting vectors, generation of gene-targeted rat ES cells and, finally, production of gene-targeted rats. The major differences between this protocol and the classical mouse gene-targeting protocol include ES cell culture methods, drug selection scheme… CONTINUE READING
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