Generating chimeric mice from embryonic stem cells via vial coculturing or hypertonic microinjection.


The generation of a fertile embryonic stem cell (ESC)-derived or F0 (100 % coat color chimerism) mice is the final criterion in proving that the ESC is truly pluripotent. Many methods have been developed to produce chimeric mice. To date, the most popular methods for generating chimeric embryos is well sandwich aggregation between zona pellucida (ZP… (More)
DOI: 10.1007/978-1-4939-1215-5_5


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