Generating Transparent Zebrafish: A Refined Method to Improve Detection of Gene Expression During Embryonic Development

  title={Generating Transparent Zebrafish: A Refined Method to Improve Detection of Gene Expression During Embryonic Development},
  author={Johnny Karlsson and Jonas von Hofsten and P-E Olsson},
  journal={Marine Biotechnology},
Abstract: In zebrafish (Danio rerio) pigmentation is initiated during embryogenesis and begins in the retinal epithelium and in the melanophores. The pigment cells develop rapidly, and within hours they constitute a prominent feature of the embryo. In order to improve signal detection by whole mount in situ hybridization, confocal microscopy, or expression of GFP, embryos may be treated with 1-phenyl 2-thiourea (PTU) during embryogenesis. PTU inhibits melanogenesis by blocking all tyrosinase… Expand
A crystal-clear zebrafish for in vivo imaging
The larval zebrafish (Danio rerio) is an excellent vertebrate model for in vivo imaging of biological phenomena at subcellular, cellular and systems levels. However, the optical accessibility ofExpand
All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish
The method is an all in one solution that enables the detection and visualization of gene expression patterns up to the late larval stage in a 3D manner without the need for tissue sectioning and offers a valuable extension for whole mount ISH by immunohistochemistry in the zebrafish field. Expand
Analysis of the retina in the zebrafish model.
Experimental approaches used in the zebrafish model to study morphogenetic transformations, cell fate decisions, and the differentiation of fine morphological features that ultimately lead to the formation of the functional vertebrate visual system are discussed. Expand
See-Thru-Gonad zebrafish line: developmental and functional validation.
See-Thru-Gonad zebrafish, a transparent line with fluorescently labeled germ cells visible throughout the life cycle, is established and its applicability is demonstrated by performing a targeted gene knockdown experiment using vivo-morpholinos (VMOs). Expand
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This chapter reviews methods used to functionally manipulate and visualize the vasculature in the zebrafish and illustrates how these methods have helped further understanding of the genetic components regulating formation and patterning of developing vessels. Expand
The rise of photoresponsive protein technologies applications in vivo: a spotlight on zebrafish developmental and cell biology
A number of fluorescent protein and optogenetic technologies have emerged that allow new ways to visualize, quantify, and perturb developmental dynamics in zebrafish. Expand
The rise of photoresponsive protein technologies applications in : a spotlight on zebrafish developmental and cell biology vivo
  • 2018
The zebrafish ( ) is a powerful vertebrate model to study cellular and Danio rerio developmental processes . The optical clarity and their amenability to in vivo genetic manipulation make zebrafish aExpand
Enhanced canonical Wnt signaling during early zebrafish development perturbs the interaction of cardiac mesoderm and pharyngeal endoderm and causes thyroid specification defects
It is indicated that BMP signaling is critically required for thyroid cell specification and cardiac mesoderm is identified as a likely source of BMP signals. Expand
Analysis of the retina in the zebrafish model.
Experimental approaches that range from cell lineage analysis to the imaging of synaptic calcium currents and atomic force microscopy are discussed, used in zebrafish to model morphogenetic events during early development of the eye primordium, cell fate decisions during retinal neurogenesis, and the differentiation and function of the many fine structural features that underlie the detection and processing of light stimuli in the eye. Expand
Enhanced in vivo-imaging in medaka by optimized anaesthesia, fluorescent protein selection and removal of pigmentation
The results indicate that eGFP and mCherry are the best conservative choices for in vivo-fluorescence experiments, when availability of well-established antibodies and nanobodies matters, and that mVenusNB and mGFPmut2 delivered highest absolute fluorescence intensities in vivo. Expand


Zebrafish pigmentation mutations and the processes of neural crest development.
Comparison of their phenotypes permits classification of these mutations according to the types of defects they cause, and these suggest which process of neural crest development is probably affected, as part of a large-scale mutagenesis screen for embryonic/early larval mutations. Expand
Stages of embryonic development of the zebrafish
A series of stages for development of the embryo of the zebrafish, Danio (Brachydanio) rerio is described, providing for flexibility and continued evolution of the staging series as the authors learn more about development in this species. Expand
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This chapter describes several alternative approaches for identifying multiple transcripts that allow visualization of the transcripts either with chromogenic substrates or different fluorochromes, particularly appropriate when coupled with the use of confocal microscopy. Expand
An analysis of melanogenesis in differentiating pigment cells of ascidian embryos.
This investigation defines two functionally distinct processes as part of melanin granulogenesis: appearance and activity of the tyrosinase enzyme, and accumulation of precursor tyrosine, which appear to be capable of independent regulation. Expand
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It is found that the basal levels of melanogenic activity in pigmented and nonpigmented cells correlate with expression of surface MSH receptors rather than with production of tyrosinase. Expand
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Results suggest that the depigmenting activity of HQ may partly be related to the ability of the compound to act as an alternate substrate of tyrosinase, thereby competing for tyrosine oxidation in active melanocytes. Expand
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