Generating Transparent Zebrafish: A Refined Method to Improve Detection of Gene Expression During Embryonic Development

@article{Karlsson2001GeneratingTZ,
  title={Generating Transparent Zebrafish: A Refined Method to Improve Detection of Gene Expression During Embryonic Development},
  author={Johnny Karlsson and Jonas von Hofsten and P-E Olsson},
  journal={Marine Biotechnology},
  year={2001},
  volume={3},
  pages={522-527}
}
Abstract: In zebrafish (Danio rerio) pigmentation is initiated during embryogenesis and begins in the retinal epithelium and in the melanophores. The pigment cells develop rapidly, and within hours they constitute a prominent feature of the embryo. In order to improve signal detection by whole mount in situ hybridization, confocal microscopy, or expression of GFP, embryos may be treated with 1-phenyl 2-thiourea (PTU) during embryogenesis. PTU inhibits melanogenesis by blocking all tyrosinase… Expand
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