Generalized Immunoassay for Autographa californica Nuclear Polyhedrosis Virus Infectivity In Vitro.

@article{Volkman1982GeneralizedIF,
  title={Generalized Immunoassay for Autographa californica Nuclear Polyhedrosis Virus Infectivity In Vitro.},
  author={Loy E. Volkman and P A Goldsmith},
  journal={Applied and environmental microbiology},
  year={1982},
  volume={44 1},
  pages={227-33}
}
A quantitative in vitro immunoassay for the infectivity of Autographa californica nuclear polyhedrosis virus was developed and performed in six different lepidopteran cell lines. The assay was not dependent upon cytopathic effect or polyhedron production, but rather upon viral antigen production and its recognition in a peroxidase-antiperoxidase staining procedure. The importance of using such an assay for accurately assessing infectivity in cell lines which produce polyhedra inefficiently was… CONTINUE READING

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