General suitability of techniques for in situ detection of apoptosis in small intestinal epithelium.

@article{Groos2003GeneralSO,
  title={General suitability of techniques for in situ detection of apoptosis in small intestinal epithelium.},
  author={Stephanie Groos and Enrico Reale and Liliana Luciano},
  journal={The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology},
  year={2003},
  volume={272 2},
  pages={503-13}
}
The present study was designed to evaluate different techniques for the in situ detection of apoptosis in human and rat small intestinal epithelium. The techniques included light microscopy (LM) and transmission electron microscopy (TEM) observation of epoxy resin-embedded tissue, scanning electron microscopy (SEM), TUNEL assay, and antibodies directed against caspase cleavage products of caspase 3, cytokeratin 18 (CK 18), and apoptotic single-strand DNA (ssDNA). All techniques, if the labeling… CONTINUE READING
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