General strategy for ordered noncovalent protein assembly on well-defined nanoscaffolds.

@article{Pille2013GeneralSF,
  title={General strategy for ordered noncovalent protein assembly on well-defined nanoscaffolds.},
  author={Jan Pille and Daniela Cardinale and No{\"e}lle Carette and Carmelo Di Primo and Jane Besong-Ndika and Jocelyne Walter and Herv{\'e} Lecoq and Mark B van Eldijk and Ferdinanda C M Smits and Sanne Schoffelen and Jan C M van Hest and Kristiina M{\"a}kinen and Thierry Michon},
  journal={Biomacromolecules},
  year={2013},
  volume={14 12},
  pages={4351-9}
}
Here we develop a novel approach allowing the noncovalent assembly of proteins on well-defined nanoscaffolds such as virus particles. The antibody-binding peptide Z33 was genetically fused to the monomeric yellow fluorescent protein and 4-coumarate:CoA-ligase 2. This Z33 "tag" allowed their patterning on the surface of zucchini yellow mosaic virus by means of specific antibodies directed against the coat protein of the virus. The approach was validated by affinity assays and correlative… CONTINUE READING