General method for quantifying base adducts in specific mammalian genes.

@article{Thomas1988GeneralMF,
  title={General method for quantifying base adducts in specific mammalian genes.},
  author={D. Thomas and A. G. Morton and V. Bohr and A. Sancar},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1988},
  volume={85 11},
  pages={
          3723-7
        }
}
  • D. Thomas, A. G. Morton, +1 author A. Sancar
  • Published 1988
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • A general method has been developed to measure the formation and removal of DNA adducts in defined sequences of mammalian genomes. Adducted genomic DNA is digested with an appropriate restriction enzyme, treated with Escherichia coli UvrABC excision nuclease (ABC excinuclease), subjected to alkaline gel electrophoresis, and probed for specific sequences by Southern hybridization. The ABC excinuclease incises DNA containing bulky adducts and thus reduces the intensity of the full-length… CONTINUE READING
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