General method for HPLC purification and sequencing of selected dsDNA gene fragments from complex PCRs generated during gene expression profiling.

Abstract

Gene expression profiling using an AFLP-based technique generates a large number of gene fragments that require identification by sequencing. The DNA fragments vary in length from about 50-500 bp. Ion-pair reversed-phase HPLC can be used to purify selected double-stranded DNA fragments that represent differentially expressed genes. The gene fragments are sequenced directly after vacuum drying of the collected HPLC fractions.

Cite this paper

@article{Wong2000GeneralMF, title={General method for HPLC purification and sequencing of selected dsDNA gene fragments from complex PCRs generated during gene expression profiling.}, author={Larissa Y Wong and Victor Belonogoff and Victoria L . Boyd and Nathan M Hunkapiller and Petra M. Casey and Shirlene N Liew and K D Lazaruk and Suzanna Baumhueter}, journal={BioTechniques}, year={2000}, volume={28 4}, pages={776-83} }