General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad.

@article{Imhoff2003GeneralBC,
  title={General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad.},
  author={Rebecca D Imhoff and Nicholas P Power and Martin Jack Borrok and Peter A. Tipton},
  journal={Biochemistry},
  year={2003},
  volume={42 14},
  pages={4094-100}
}
Urate oxidase catalyzes the oxidation of urate without the involvement of any cofactors. The gene encoding urate oxidase from Bacillus subtilis has been cloned and expressed, and the enzyme was purified and characterized. Formation of the urate dianion is believed to be a key step in the oxidative reaction. Rapid-mixing chemical quench studies provide evidence that the dianion is indeed an intermediate; at 15 degrees C the dianion forms within the mixing time of the rapid-quench instrument, and… CONTINUE READING

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