General anesthetics modulate GABA receptor channel complex in rat dorsal root ganglion neurons

@article{Nakahiro1989GeneralAM,
  title={General anesthetics modulate GABA receptor channel complex in rat dorsal root ganglion neurons},
  author={Masanobu Nakahiro and Jay Z. Yeh and Edward A. Brunner and Toshio Narahashi},
  journal={The FASEB Journal},
  year={1989},
  volume={3},
  pages={1850 - 1854}
}
The effects of halothane, isoflurane, and enflurane on ionic currents induced by bath application of γ‐aminobutyric acid (GABA) were studied with the rat dorsal root ganglion neurons maintained in primary culture. The whole‐cell patch clamp technique was used to record the current. In normal neurons before exposure to anesthetics, GABA at low concentrations (1‐3 times 10−6 M) induced a small sustained inward current. At higher concentrations (3 times 10−5 M‐1 times 10−3 M), GABA induced a large… 
Effects of Small Concentrations of Volatile Anesthetics on Action Potential Firing of Neocortical Neurons In Vitro
TLDR
Evidence is provided that halothane, isoflurane, and enflurane reduced spontaneous action potential firing of neocortical neurons in cultured brain slices mainly by increasing GABAA‐mediated synaptic inhibition.
Local anesthetics reduce the inhibitory neurotransmitter-induced current in dissociated hippocampal neurons of the rat.
TLDR
Benzocaine, a neutral local anesthetic at physiological pH, decreased the Gly-ICl more potently than lidocane, while QX314, a permanently charged quaternary derivative of lidocaine produced a much smaller inhibition, thereby indicating that the neutral form of local anesthetics is more effective in reducing the Gly -ICl.
Dimethyl sulfoxide (DMSO) blocks GABA-induced current in rat dorsal root ganglion neurons
TLDR
The results suggest that DMSO suppressed the currents by interacting with GABA receptor-Cl- channel complex protein rather than by affecting the lipid-bilayer of the cell membrane.
Volatile anesthetics gate a chloride current in postnatal rat hippocampal neurons
  • J. Yang, K. Isenberg, C. Zorumski
  • Biology, Medicine
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1992
TLDR
It is likely that drugs with both direct GABA agonist and GABA modulatory properties will produce overall depression of the central nervous system by increasing the normal inhibitory synaptic influence and by directly hyperpolarizing neurons.
Stereoselective and non‐stereoselective actions of isoflurane on the GABAA receptor
TLDR
The sustained residual current remaining after exposure of neurones to a desensitizing concentration of GABA was inhibited non‐stereoselectively, but only at high concentrations of isoflurane, and the inhibition was barely significant at the EC50 concentration for general anaesthesia.
Sevoflurane modulates both GABAA and GABAB receptors in area CA1 of rat hippocampus.
TLDR
It is demonstrated that sevoflurane at clinical concentrations activated both GABAA- and GABAB-mediated inhibitions in area CA1 of the hippocampus, and that seVofl Lurane and GABA agonists acted on different domains on the GAB AA and GABA B receptors, respectively.
Modulation of neuronal nicotinic acetylcholine receptors by halothane in rat cortical neurons.
TLDR
Halothane block of nnAChRs is deemed to play an important role in anesthesia via a direct action on the receptor and an indirect action to suppress transmitter release.
Potentiation by sevoflurane of the γ‐aminobutyric acid‐induced chloride current in acutely dissociated CA1 pyramidal neurones from rat hippocampus
TLDR
It is concluded that Sev acts on the GABAA receptor complex mimicking the GABA‐induced chloride current at high concentrations, which may result in the depressing of postsynaptic excitability and may, at least in part, underlie the anaesthetic action of Sev.
Effects of the volatile anesthetic enflurane on spontaneous discharge rate and GABA(A)-mediated inhibition of Purkinje cells in rat cerebellar slices.
TLDR
Results suggest that enflurane-induced membrane hyperpolarizations, as well as the reduction of spike rates, were partly caused by an increase in synaptic inhibition.
The effects of general anesthetics on excitatory and inhibitory synaptic transmission in area CA1 of the rat hippocampus in vitro.
TLDR
The findings indicate that pentobarbital and propofol produce inhibitory actions due to enhancement in the GABA(A) receptor; that ketamine reduces NMDA receptor-mediated responses and enhances GABA( a) receptor- mediated responses; and that halothane and isoflurane modulate GABA(a), NMDA, and non-NMDA receptor -mediated synaptic transmission.
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INTERACTION OF PENTOBARBITONE AND γ‐AMINOBUTYRIC ACID ON MAMMALIAN SYMPATHETIC GANGLION CELLS
1 Interactions of bath‐applied pentobarbitone and γ‐aminobutyric acid (GABA) on neurones in isolated superior cervical ganglia of the rat have been examined with intracellular microelectrodes. 2
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