General LC-MS/MS method approach to quantify therapeutic monoclonal antibodies using a common whole antibody internal standard with application to preclinical studies.

@article{Li2012GeneralLM,
  title={General LC-MS/MS method approach to quantify therapeutic monoclonal antibodies using a common whole antibody internal standard with application to preclinical studies.},
  author={Hongyan Li and Robert Ortiz and Linh Khanh Tran and Michael W J Hall and Christopher S Spahr and Ken Walker and John Laudemann and Sterling D. Miller and Hossein Salimi-Moosavi and Jean Wenqing. Lee},
  journal={Analytical chemistry},
  year={2012},
  volume={84 3},
  pages={
          1267-73
        }
}
Ligand binding assays (LBAs) are widely used for therapeutic monoclonal antibody (mAb) quantification in biological samples. Major limitations are long method development times, reagent procurement, and matrix effects. LC-MS/MS methods using signature peptides are emerging as an alternative approach, which typically use a stable isotope labeled signature peptide as the internal standard (IS). However, a new IS has to be generated for every candidate, and the IS may not correct for variations at… CONTINUE READING
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