General Approach to the Immobilization of Glycoenzyme Chains Inside Calcium Alginate Beads for Bioassay.


A general method to obtain the efficient entrapment of mixtures of glycoenzymes in calcium alginate hydrogel is proposed in this paper. As a proof of principle, three glycoenzymes acting in series (trehalase, glucose oxidase, and horseradish peroxidase) have been coimmobilized in calcium alginate beads. The release of the enzymes from the hydrogel mesh… (More)
DOI: 10.1021/acs.analchem.5b02636


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