General, Label-Free Method for Determining K(d) and Ligand Concentration Simultaneously.

@article{JalaliYazdi2015GeneralLM,
  title={General, Label-Free Method for Determining K(d) and Ligand Concentration Simultaneously.},
  author={Farzad Jalali-Yazdi and Terry T. Takahashi and Richard W Roberts},
  journal={Analytical chemistry},
  year={2015},
  volume={87 23},
  pages={11755-62}
}
Some of the most commonly used affinity reagents (e.g., antibodies) are often developed and used in conditions where their input concentrations ([L]0) and affinities (K(d)) are not known. Here, we have developed a general approach to determine both [L]0 and K(d) values simultaneously for affinity reagents (small molecules, proteins, and antibodies). To do… CONTINUE READING