Gene trapping of the Arabidopsis genome with a firefly luciferase reporter.

  title={Gene trapping of the Arabidopsis genome with a firefly luciferase reporter.},
  author={Yoshiharu Y. Yamamoto and Yumi Tsuhara and Kazuhito Gohda and Kumiko Suzuki and Minami Matsui},
  journal={The Plant journal : for cell and molecular biology},
  volume={35 2},
Experiments with gene-trap vectors containing the firefly luciferase (LUC) reporter genes were carried out with the aim of analyzing functions of the Arabidopsis genome. Studies with protein fusion-type trap vectors as well as an internal ribosome entry site (IRES)-assisted non-fusion-type vector revealed that both types of vectors were suitable for gene trapping in Arabidopsis, although there were some differences in trapping efficiencies. The established trap lines were subjected to analyses… CONTINUE READING
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