Gene transfer into insect brain and cell-specific expression of bombyxin gene

@article{Moto1999GeneTI,
  title={Gene transfer into insect brain and cell-specific expression of bombyxin gene},
  author={Ken’ichi Moto and Salah Eldin Abdel Salam and Sho Sakurai and Masafumi Iwami},
  journal={Development Genes and Evolution},
  year={1999},
  volume={209},
  pages={447-450}
}
 A transgene reporter consisting of the bombyxin gene promoter and the green fluorescent protein coding region was introduced into intact brains of the silkworm Bombyx mori by in vitro electroporation. After in vitro culture of the brains, the fluorescence derived from the introduced reporter gene was observed in all cases in eight neurosecretory cells that had previously been identified as bombyxin-producing cells (BPCs). Although the fluorescence was not always observed in all cells, it was… CONTINUE READING

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