Gene transfer into Solanum tuberosum via Rhizobium spp.

  title={Gene transfer into Solanum tuberosum via Rhizobium spp.},
  author={Toni Wendt and Fiona M. Doohan and Dominik Winckelmann and Ewen Mullins},
  journal={Transgenic Research},
Agrobacterium tumefaciens-mediated transformation (ATMT) is the preferred technique for gene transfer into crops. A major disadvantage of the technology remains the complexity of the patent landscape that surrounds ATMT which restricts its use for commercial applications. An alternative system has been described (Broothaerts et al. in Nature 433:629-633, 2005) detailing the propensity of three rhizobia to transform the model crop Arabidopsis thaliana, the non-food crop Nicotiana tabacum and, at… 

Production of Phytophthorainfestans-resistant potato (Solanum tuberosum) utilising Ensifer adhaerens OV14

The potential of Ensifer-mediated transformation (EMT) as a novel platform for the high frequency generation of transgenic potato is confirmed and resistant genotypes were confirmed by associated molecular analysis and resistant phenotypes demonstrated by the development of hypersensitive lesions on inoculated leaf tissue post-pathogen inoculation.

A robust genetic transformation protocol to obtain transgenic shoots of Solanum tuberosum L. cultivar ‘Kufri Chipsona 1’

The genetic transformation of plants is an important biotechnological tool used for crop improvement for many decades. The present study was focussed to investigate various factors affecting genetic

Pathways of DNA Transfer to Plants from Agrobacterium tumefaciens and Related Bacterial Species.

This work states that closely related bacterial species, such as rhizobia, are able to transfer DNA to host plant cells when they are provided with Agrobacterium DNA transfer machinery and T-DNA, which likely reflects the very wide host range of Agrobacteria spp.

Capability of the plant-associated bacterium, Ensifer adhaerens strain OV14, to genetically transform its original host Brassica napus

The successful transformation of the commercial B. napus cultivar Delight is reported, highlighting the expanding host range of Ensifer-mediated transformation by confirming the ability of the symbiont Ensifer adhaerens OV14 to genetically engineer its original host.

A Functional Bacterium-to-Plant DNA Transfer Machinery of Rhizobium etli

It is shown that Rhizobium etli can mediate functional DNA transfer and stable genetic transformation of plant cells, when provided with a plasmid containing a T-DNA segment.

State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential

Current challenges and opportunities concerning potato genetic engineering strategies developed to date are discussed, their critical parameters and constrains are described, and the potential application of the available tools for functional analyses or biotechnological purposes are addressed.

Transcriptional Activation of Virulence Genes of Rhizobium etli

The transcriptional regulation and induction of virulence genes in R. etli are explored and show similarities to and differences from those of their A. tumefaciens counterparts, contributing to an understanding and a comparison of these two systems.

Insights into the transcriptomic response of the plant engineering bacterium Ensifer adhaerens OV14 during transformation

This is the first study to characterise the whole transcriptomic response of a plant engineering bacterium in the presence of plant tissues and provides a novel insight into prokaryotic genetic processes that support T-DNA transfer.

Applications of biotechnology and genomics in potato improvement.

The recent determination of the potato genome sequence has presented new opportunities for genomewide assays to provide tools for gene discovery and enabling the development of robustly unique marker haplotypes spanning QTL regions.

Genome sequence of Ensifer adhaerens OV14 provides insights into its ability as a novel vector for the genetic transformation of plant genomes

The sequencing and analysis of the OV14 genome increases the understanding of T-DNA transfer by non-Agrobacterium species and creates a platform for the continued improvement of Ensifer-mediated transformation (EMT).



Establishment of a highly efficient Agrobacterium tumefaciens-mediated leaf disc transformation method for Broussonetia papyrifera

Using this protocol, transgenic B. papyrifera plants containing desirable new genes can be obtained in approximately 3 months with a transformation frequency as high as 44%.

Agrobacterium-mediated transformation protocol to overcome necrosis in elite AustralianBrassica juncea lines

A Agrobacterium-mediated transformation protocol to overcome necrosis in elite AustralianB.

The Rpi-blb2 gene from Solanum bulbocastanum is an Mi-1 gene homolog conferring broad-spectrum late blight resistance in potato.

The positional cloning of the Solanum bulbocastanum-derived Rpi-blb2 gene, which even when present in a potato background confers broad-spectrum late blight resistance, is described, indicating that intrachromosomal recombination or unequal crossing over has played an important role in the evolution of the RPI-blB2 locus.

Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight

The cloning of the major resistance gene RB in S. bulbocastanum is reported by using a map-based approach in combination with a long-range (LR)-PCR strategy, demonstrating that LR-PCR is a valuable approach to isolate genes that cannot be maintained in the bacterial artificial chromosome system.

Gene transfer to plants by diverse species of bacteria

It is shown that several species of bacteria outside the Agrobacterium genus can be modified to mediate gene transfer to a number of diverse plants, which may lead to new uses of natural bacteria–plant interactions to achieve plant transformation.

Direct gene transfer in potato: A comparison of particle bombardment of leaf explants and PEG-mediated transformation of protoplasts

No correlation between the number of gene insertions and gene expression levels was found, suggesting that multiple insertions may have little or no effect on transgene expression.

Accumulation of vitamin E in potato (Solanum tuberosum) tubers

The results indicate that physiological and regulatory constraints may be the most limiting factors for tocopherol accumulation in potato tubers and should reveal approaches to more effectively engineer crops with enhanced toCopherol content.

Transformation of a large number of potato varieties: genotype-dependent variation in efficiency and somaclonal variability

Field experiments, conducted over a number of years, using plants with different degrees of antisense effect showed that most transformants would have a decreased yield and starch content as determined by specific gravity measurements, however, these negative effects can be overcome by selecting the proper transgenic plants.

Transformation of Tobacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector System.

Using a binary tumor-inducing (Ti) plasmid vector system, several plant species were transformed with a kanamycin resistance marker (neomycin phosphotransferase gene) and it is demonstrated that a wild type Ti plasmids can be used as a helper to obtain a transformed plant.

GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

GUS is very stable, and tissue extracts continue to show high levels of GUS activity after prolonged storage, and Histochemical analysis has been used to demonstrate the localization of gene activity in cells and tissues of transformed plants.