Gene transduction in human monocyte-derived dendritic cells using lentiviral vectors.

@article{Satoh2013GeneTI,
  title={Gene transduction in human monocyte-derived dendritic cells using lentiviral vectors.},
  author={Takeshi Satoh and Nicolas Manel},
  journal={Methods in molecular biology},
  year={2013},
  volume={960},
  pages={401-409}
}
Monocyte-derived dendritic cells (MDDCs) are widely used in the field of human immunology. Although a variety of gene delivery procedures have been used in MDDC, it has remained difficult to achieve robust gene transductions. In this chapter, we describe a procedure for high efficiency gene transduction in human MDDCs using lentiviral vectors. Gene transduction based on HIV-1-derived lentiviral vectors is restricted at the level of reverse transcription by the cellular protein SAMHD1 in MDDCs… CONTINUE READING