Gene transcription in a temperature-sensitive mutant of RNA polymerase II.

Abstract

We have studied the transcription rates and the steady-state levels of cytoplasmic mRNA of selected genes in tsAF8 cells, a temperature-sensitive mutant of RNA polymerase II. At the restrictive temperature, the transcription rates decrease uniformly for all genes examined, while the corresponding decreases in steady-state levels of cytoplasmic mRNA vary for individual genes. tsAF8 cells can be advantageously used to study the half-line of mRNA without the use of perturbing drugs.

Cite this paper

@article{Jaskulski1988GeneTI, title={Gene transcription in a temperature-sensitive mutant of RNA polymerase II.}, author={Dariusz Jaskulski and Renato Baserga}, journal={APMIS. Supplementum}, year={1988}, volume={2}, pages={82-9} }