Gene transcript amplification from cell lysates in continuous-flow microfluidic devices

@article{Gonzlez2007GeneTA,
  title={Gene transcript amplification from cell lysates in continuous-flow microfluidic devices},
  author={Asensio Gonz{\'a}lez and Doina Ciobanu and Michael Sayers and Noel Sirr and T. Dalton and Mark R. D. Davies},
  journal={Biomedical Microdevices},
  year={2007},
  volume={9},
  pages={729-736}
}
Continuous-flow analysis, where samples circulate encapsulated in a carrier fluid is an attractive alternative to batch processing for high-throughput devices that use the polymerase chain reaction (PCR). Challenges of continuous-flow prototypes include the hydrodynamic and biological incompatibility of the carrier fluid, microchannel fouling, sample carryover and the integration of a nucleic acid extraction and reverse transcription step. We tested two homemade, continuous-flow thermocycler… 
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