Gene targeting with zinc finger nucleases to produce cloned eGFP knockout pigs.

@article{Whyte2011GeneTW,
  title={Gene targeting with zinc finger nucleases to produce cloned eGFP knockout pigs.},
  author={Jeffrey J. Whyte and Jianguo Zhao and Kevin Dale Wells and Melissa S. Samuel and Kristin M Whitworth and Eric Michael Walters and Maurice Harold Laughlin and Randall S Prather},
  journal={Molecular reproduction and development},
  year={2011},
  volume={78 1},
  pages={
          2
        }
}
We report using zinc finger nucleases (ZFNs) and somatic cell nuclear transfer (SCNT) to generate pigs with a knockout (KO) mutation of an enhanced green fluorescent protein (eGFP) transgene. ZFNs are synthetic modular proteins composed of a FokI endonuclease domain linked to a sequence-specific zinc finger DNA-binding domain. Pairs of ZFNs bind to the target region, allowing FokI dimerization and subsequent DNA cleavage. Mutation of an eGFP transgene by using ZFNs has been demonstrated in rats… CONTINUE READING
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