Gene targeting of DT-diaphorase in mouse embryonic stem cells: establishment of null mutant and its mitomycin C-resistance.


It is generally accepted that DT-diaphorase is primarily involved in the detoxification of quinone compounds and is capable of metabolically activating some cancer chemotherapeutic quinones including mitomycin C. However, these conclusions have mainly been drawn from the experiments using the DT-diaphorase inhibitor, dicoumarol. To understand directly the… (More)