Gene targeting in a nonhomologous end joining deficient Hypocrea jecorina.

  title={Gene targeting in a nonhomologous end joining deficient Hypocrea jecorina.},
  author={Zhang Guangtao and Lukas Hartl and Andr{\'e} J Schuster and {\vS}tefan Pol{\'a}k and Monika Schmoll and Tianhong Wang and Verena Seidl and Bernhard Seiboth},
  journal={Journal of biotechnology},
  volume={139 2},
The industrially applied ascomycete Hypocrea jecorina (synonym: Trichoderma reesei) exhibits a low rate of exogenous DNA integration by homologous recombination (HR). This hinders the high-throughput generation of strains by gene replacement and is therefore impeding systematic functional gene analyses towards, e.g. strain improvement for protein or enzyme production. To increase the rate of HR events during fungal transformation we identified and deleted the orthologue of the human KU70 in H… CONTINUE READING


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