Gene targeting in a HUES line of human embryonic stem cells via electroporation.

@article{Ruby2009GeneTI,
  title={Gene targeting in a HUES line of human embryonic stem cells via electroporation.},
  author={Katherine M Ruby and Binhai Zheng},
  journal={Stem cells},
  year={2009},
  volume={27 7},
  pages={1496-506}
}
Genetic modification is critical for achieving the full potential of human embryonic stem (ES) cells as a tool for therapeutic development and for basic research. Targeted modifications in human ES cells have met with limited success because of the unique culture conditions for many human ES cell lines. The HUES lines of human ES cells were developed for ease of manipulation and are gaining increased utility in stem cell research. We tested conditions for gene targeting via electroporation in… CONTINUE READING

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