Gene splicing and mutagenesis by PCR-driven overlap extension

@article{Heckman2007GeneSA,
  title={Gene splicing and mutagenesis by PCR-driven overlap extension},
  author={Karin L Heckman and Larry R. Pease},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={924-932}
}
  • Karin L Heckman, Larry R. Pease
  • Published 2007
  • Medicine, Biology
  • Nature Protocols
  • Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and gene splicing. Initial PCRs generate overlapping gene segments that are then used as template DNA for another PCR to create a full-length product. Internal primers generate overlapping, complementary 3′ ends on the intermediate segments and introduce nucleotide substitutions, insertions or deletions for site-directed mutagenesis, or for gene splicing, encode the nucleotides found at… CONTINUE READING

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    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 33 REFERENCES
    The "megaprimer" method of site-directed mutagenesis.
    • 1,063
    Hybridization Analysis of DNA Blots
    • 10
    Using Synthetic Oligonucleotides as Probes
    • 14