Gene-specific silencing induced by parallel complementary RNA in Pseudomonas aeruginosa


To investigate whether parallel complementary RNA (pRNA) could induce gene-specific silencing in Pseudomonas aeruginosa, pRNA of the mexA gene was expressed in it. Compared to the control strains, the strain expressing pRNA of mexA showed a 50% decrease in minimum inhibitory concentrations (MICs) of several antimicrobial agents and a twofold increase in the initial accumulation rate of ethidium bromide, all of which are substrates of the MexAB-OprM efflux pump. These results suggest that gene-specific silencing was induced by pRNA. This is the first time that such a route for gene silencing has been reported in a bacterium other than Escherichia coli. Gene-specific silencing induced by pRNA may be useful as a novel biotechnology tool for gene regulation in prokaryotes.

DOI: 10.1007/s10529-009-0046-x

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@article{Liu2009GenespecificSI, title={Gene-specific silencing induced by parallel complementary RNA in Pseudomonas aeruginosa}, author={Yi Shuang Liu and Yue Zhang and Li Xia Yang and Tian Yao and Chun Ling Xiao}, journal={Biotechnology Letters}, year={2009}, volume={31}, pages={1571-1575} }