Gene-specific dye bias in microarray reference designs.

@article{Dombkowski2004GenespecificDB,
  title={Gene-specific dye bias in microarray reference designs.},
  author={Alan A. Dombkowski and Bryan J. Thibodeau and Susan L Starcevic and Raymond F. Novak},
  journal={FEBS letters},
  year={2004},
  volume={560 1-3},
  pages={
          120-4
        }
}

• Alan A. Dombkowski, Bryan J. Thibodeau, +1 author Raymond F. Novak
• Published in FEBS letters 2004
• DOI:10.1016/S0014-5793(04)00083-3

The most widely used microarray experiment design includes the use of a reference standard. Comparisons of gene expression between samples are facilitated because each sample is directly measured against the reference standard, using two fluorescent dyes. Numerous reports indicate that some genes incorporate the two commonly used dyes with different efficiencies, contributing to inaccurate data. However, it is widely assumed that these effects will not corrupt results if the reference standard… CONTINUE READING

View on PubMed

Open Access

Save to Library

Create Alert

Cite