Gene silencing using a heat-inducible RNAi system in Arabidopsis.

  title={Gene silencing using a heat-inducible RNAi system in Arabidopsis.},
  author={Fr{\'e}d{\'e}ric G. Masclaux and Martine Charpenteau and Taku Takahashi and Rafael Pont-Lezica and J. P. Galaud},
  journal={Biochemical and biophysical research communications},
  volume={321 2},
Controlling gene expression during plant development is an efficient tool to explore gene function. In this paper, we describe a gene expression system driven by a heat-shock gene promoter (HSP18.2), to trigger the expression of an intron-containing inverted-repeat. RNA interference became a powerful way for gene functional analysis by reverse genetic approaches. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability. Inducible… CONTINUE READING