Gene silencing in Xenopus laevis by DNA vector-based RNA interference and transgenesis

Abstract

A vector-based RNAi expression system was developed using the Xenopus tropicalis U6 promoter, which transcribes small RNA genes by RNA polymerase III. The system was first validated in a Xenopus laevis cell line, designing a short hairpin DNA specific for the GFP gene. Co-transfection of the vector-based RNAi and the GFP gene into Xenopus XR1 cells… (More)
DOI: 10.1038/sj.cr.7310013

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