Gene silencing by RNA interference in the koji mold Aspergillus oryzae.

  title={Gene silencing by RNA interference in the koji mold Aspergillus oryzae.},
  author={O. Yamada and Ryoko Ikeda and Yuka Ohkita and Risa Hayashi and Kazutoshi Sakamoto and Osamu Akita},
  journal={Bioscience, biotechnology, and biochemistry},
  volume={71 1},
We found the orthologous genes required for RNA interference (RNAi) in the Aspergillus oryzae genome database, and constructed a set of tools for gene silencing using RNAi in A. oryzae. This system utilizes compatible restriction enzyme sites so that only a single target gene fragment is required to create the hairpin RNA cassette. For ease of handling, we also separated the construction of the hairpin RNA cassette for the target gene from its subsequent introduction into the expression vector… CONTINUE READING
17 Citations
32 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 17 extracted citations


Publications referenced by this paper.
Showing 1-10 of 32 references

Redundancy of the two dicer genes in transgene-induced posttranscriptional gene silencing in Neurospora crassa

  • C. Catalanotto, M. Palotta, +4 authors C. Cogoni
  • Mol. Cell. Biol.,
  • 2004

Transformation system for Aspergillus oryzae with double auxotrophic mutations , niaD and sC

  • O. Yamada, B. R. Lee, K. Gomi
  • Biosci . Biotechnol . Biochem .
  • 2004

Similar Papers

Loading similar papers…