Gene sequences of the pcpB gene of pentachlorophenol-degrading Sphingomonas chlorophenolica found in nondegrading bacteria.

@article{Saboo1998GeneSO,
  title={Gene sequences of the pcpB gene of pentachlorophenol-degrading Sphingomonas chlorophenolica found in nondegrading bacteria.},
  author={V M Saboo and Michael A. Gealt},
  journal={Canadian journal of microbiology},
  year={1998},
  volume={44 7},
  pages={667-75}
}
Bacteria isolated from a pentachlorophenol (PCP) contaminated site grew in the presence of 50 micrograms PCP/mL but were not able to degrade it in either liquid medium or the presence of 1% sterile potting soil as a solid support. Probes developed using the gene sequence of PCP-4-monooxygenase (pcpB) from Sphingomonas chlorophenolica sp.nov hybridized to two separate isolates. Identification based on fatty acid methyl ester profiles (Sherlock), substrate utilization (BIOLOG), and 16S rRNA… CONTINUE READING