Gene repair in mammalian cells is stimulated by the elongation of S phase and transient stalling of replication forks.

@article{Brachman2005GeneRI,
  title={Gene repair in mammalian cells is stimulated by the elongation of S phase and transient stalling of replication forks.},
  author={Erin E Brachman and Eric B. Kmiec},
  journal={DNA repair},
  year={2005},
  volume={4 4},
  pages={445-57}
}
The repair of point mutations directed by modified single-stranded DNA oligonucleotides is dependent on the activity of proteins involved in homologous recombination (HR). As a consequence, factors that stimulate homologous recombination, such as double strand breaks, can impact the frequency with which repair occurs. Here, we report that the stalling of replication forks can also activate the gene repair pathway and lead to an enhanced level of nucleotide exchange. The mammalian cell line, DLD… CONTINUE READING