• DOI:10.2135/CROPSCI1997.0011183X003700020049X
• Corpus ID: 83567365

Gene pool classification of common bean landraces from Chile based on RAPD and morphological data

@article{Johns1997GenePC,
  title={Gene pool classification of common bean landraces from Chile based on RAPD and morphological data},
  author={Mitrick A. Johns and Paul W. Skroch and James Nienhuis and Patricio Hinrichsen and G. Bascur and Carlos Mu{\~n}oz-Schick},
  journal={Crop Science},
  year={1997},
  volume={37},
  pages={605-613}
}

• M. Johns, P. Skroch, +3 authors Carlos Muñoz-Schick
• Published 1 March 1997
• Biology
• Crop Science

Landraces of commonb ean (Phaseolus vulgaris L.) from Chile are
a phenotypically diverse group whose relationship to the two major
gene pools (Andeana nd Mesoamericani)s unclear. The genetic composition
of 69 Chileanl andraces, 15 commerciaclu itivars growni n Chile,
and 11 previously characterized check accessions was examined using
RAPDs (random amplified polymorphic DNA). The 25 primers used
generated 106 polymorphic bands. A genetic distance (GD) matrix
based on simple matching of RAPD phenotypes was converted to a
two dimensional plot using multidimensional scaling (MDS). Two
major clusters, encompassing most of the accessions, were apparent
on the resulting plot and were identified with the Andean and the
Mesoamericagne ne pools. No obvious signs of introgression or hybridization
between gene pools were observed. Clustering analysis using
bootstrap samples of the RAPD bands showed that as few as 20
randomlyc hosen bands could classify the accessions into the correct
gene pools nearly as well as all 106 bands. In general, the Andean
landraces were collected at more northerly latitudes and higher altitudes
than the Mesoamerican landraces. Morphological data were
collected for 63 landraces for seven numerical traits and 13 categorical
traits. The morphological data used were to create a distance matrix
and analyzed as with the RAPDd ata, but no clear separation of the
groups was seen. Accessions from the two groups identified by RAPDs
differed significantly for 11 of the 20 traits examined. It was concluded
that by RAPDst, he Chilean landraces could be readily classified into
the Andeana nd Mesoamericang ene pools. Morphological traits were
less effective at classification.