Gene pathway analysis of the mechanism by which the Rho-associated kinase inhibitor Y-27632 inhibits apoptosis in isolated thawed human embryonic stem cells.

@article{Ichikawa2012GenePA,
  title={Gene pathway analysis of the mechanism by which the Rho-associated kinase inhibitor Y-27632 inhibits apoptosis in isolated thawed human embryonic stem cells.},
  author={Hinako Ichikawa and Naohiro Nakata and Youichi Abo and Sakiko Shirasawa and Tadayuki Yokoyama and Susumu Yoshie and Fengming Yue and Daihachiro Tomotsune and Katsunori Sasaki},
  journal={Cryobiology},
  year={2012},
  volume={64 1},
  pages={12-22}
}
Cryopreservation is an essential technique in basic research and clinical applications of human embryonic stem (hES) cells. Cryopreserved hES cells are fragile and undergo post-thaw apoptosis. We performed gene pathway analysis on cryopreserved and thawed hES cells to examine the effect of Y-27632, a Rho-associated kinase (ROCK) inhibitor, on apoptosis and associated molecular events. Y-27632 was added to the cryopreservation solution and/or the post-thaw medium of two hES cell lines (KhES-1… CONTINUE READING
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