Gene-mutation assays in lambda lacZ transgenic mice: comparison of lacZ with endogenous genes in splenocytes and small intestinal epithelium.

@article{Delft1998GenemutationAI,
  title={Gene-mutation assays in lambda lacZ transgenic mice: comparison of lacZ with endogenous genes in splenocytes and small intestinal epithelium.},
  author={Joost H. M. van Delft and Anneke M. C. Bergmans and Floris van Dam and Ad D. Tates and Louisa Howard and Douglas J Winton and Robert A. Baan},
  journal={Mutation research},
  year={1998},
  volume={415 1-2},
  pages={85-96}
}
Comparison of results derived from transgenic animal gene-mutation assays with those from mutation analyses in endogenous genes is an important step in the validation of the former. We have used lambda lacZ transgenic mice to study alkylation-induced mutagenesis in vivo in (a) lacZ and hprt in spleen cells, and (b) lacZ and Dlb-I in small intestine from lambda lacZ+/0/Dlb-Ia/b mice. Induction of mutations by ethyl- and methylnitrosourea (ENU, MNU) and ethyl methanesulphonate (EMS) was… CONTINUE READING