Gene introduction into mouse blastocysts via "pricking".

@article{Sato1993GeneII,
  title={Gene introduction into mouse blastocysts via "pricking".},
  author={Masahiro Sato and Norihiro Tada and Reiko Iwase and Egon P. Amann},
  journal={Molecular reproduction and development},
  year={1993},
  volume={34 4},
  pages={349-56}
}
It is a well-known phenomenon that cultured mammalian cells that have been pricked in the presence of foreign DNA can be transformed. This micromanipulation 'pricking' technique was applied to mouse blastocysts to determine whether uptake of exogenous DNA would occur in the embryos. The middle region of the inner cell mass (ICM) was pricked three times in each blastocyst in a medium containing a linearized plasmid DNA. When the 60 treated blastocysts were transferred to the uterine horns of… CONTINUE READING