Gene insertion and replacement in Schizosaccharomyces pombe mediated by the Streptomyces bacteriophage φC31 site-specific recombination system

@article{Thomason2001GeneIA,
  title={Gene insertion and replacement in Schizosaccharomyces pombe mediated by the Streptomyces bacteriophage φC31 site-specific recombination system},
  author={Larry Thomason and Richard Calendar and David Ow},
  journal={Molecular Genetics and Genomics},
  year={2001},
  volume={265},
  pages={1031-1038}
}
The site-specific recombination system used by the Streptomyces bacteriophage φC31 was tested in the fission yeast Schizosaccharomyces pombe. A target strain with the phage attachment site attP inserted at the leu1 locus was co-transformed with one plasmid containing the bacterial attachment site attB linked to a ura4 + marker, and a second plasmid expressing the φC31 integrase gene. High-efficiency transformation to the Ura+ phenotype occurred when the integrase gene was expressed. Southern… CONTINUE READING

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