Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus

@article{Witte2014GeneIU,
  title={Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus},
  author={Hanh Witte and Eduardo Verde Moreno and Christian R{\"o}delsperger and Jungeun Kim and Jin-Soo Kim and Adrian Streit and Ralf J. Sommer},
  journal={Development Genes and Evolution},
  year={2014},
  volume={225},
  pages={55-62}
}
The diplogastrid nematode Pristionchus pacificus is a nematode model system for comparative studies to Caenorhabditis elegans and integrative evolutionary biology aiming for interdisciplinary approaches of evo-devo, population genetics, and ecology. For this, fieldwork can be combined with laboratory studies, and P. pacificus has a well-developed methodological toolkit of forward genetics, whole genome sequencing, DNA-mediated transformation, and various –omics platforms. Here, we establish… CONTINUE READING
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