Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis.

  title={Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis.},
  author={Jean Jacques Godon and C. Delorme and Jacek Bardowski and Marc C. Chopin and Stanislas Dusko Ehrlich and Pierre Renault},
  journal={Journal of bacteriology},
  volume={175 14},
The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The sequence is 99% homologous to that of the prototroph NCDO2118, which was determined previously. Two nonsense mutations and two small deletions were found in the auxotroph sequence, which might explain the… CONTINUE READING


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